Springer Nature
Browse

Additional file 10 of Identification of African swine fever virus-like elements in the soft tick genome provides insights into the virus’ evolution

Download (13.89 kB)
dataset
posted on 2020-10-09, 03:44 authored by Jan H. Forth, Leonie F. Forth, Samantha Lycett, Lesley Bell-Sakyi, Günther M. Keil, Sandra Blome, Sébastien Calvignac-Spencer, Antje Wissgott, Johannes Krause, Dirk Höper, Helge Kampen, Martin Beer
Additional file 10: Table S8. qRT-PCR results of Ornithodoros ticks experimentally infected with different ASFV-genotype isolate. Shown are ASFV-P72 transcript-specific Cq-values of third nymphal stage ticks fed with defibrinated pig blood, containing either 1 x 104 HAU/ml or 1 x 106 HAU/ml ASFV-ken.rie1 (GT X) (A-D), 1 x 105 HAU/ml ASFV-Ken06.bus (GT IX) (E-F) or 1 x 104 HAU/ml ASFV-Sardinia (GT I) (G-H). Due to the limited number of field ticks available and feeding under artificial conditions, fifteen Ornithodoros porcinus ticks were collected in each of three experiments and ten in two experiments while for the laboratory-reared Ornithodoros moubata, twenty-five individuals were collected in each of three experiments and fifteen in one experiment. All samples were stored at – 80 °C until RNA-extraction and ASFV transcript-specific qRT-PCR analysis as described in the ‘Material and methods’ section.

Funding

Biotechnology and Biological Sciences Research Council (GB) Biotechnology and Biological Sciences Research Council Max-Planck-Gesellschaft Horizon 2020 (COMPARE) Bundesministerium für Bildung und Forschung

History

Usage metrics

    BMC Biology

    Categories

    Exports

    RefWorks
    BibTeX
    Ref. manager
    Endnote
    DataCite
    NLM
    DC