13068_2017_966_MOESM9_ESM.pdf (189.18 kB)
MOESM9 of Transcriptomic profiling and genetic analyses reveal novel key regulators of cellulase and xylanase gene expression in Penicillium oxalicum
journal contribution
posted on 2017-11-22, 05:00 authored by Yu-Si Yan, Shuai Zhao, Lu-Sheng Liao, Qi-Peng He, Ya-Ru Xiong, Long Wang, Cheng-Xi Li, Jia-Xun FengAdditional file 9: Figure S5. Confirmation analysis of the complementary strains. Targeted complementary genes including PoxCxrA, PoxCxrB and PoxNsdD were amplified using primer pairs CxrA-CDS-F/CxrA-CDS-R, CxrB-CDS-F/CxrB-CDS-R and NsdD-CDS-F/NsdD-CDS-R. Bleomycin resistance gene was amplified using primer pair Ble-F/Ble-R. M, 1-kb DNA marker; lane 1, complementary strain; lane 2, ÎPoxKu70; lane 3, corresponding deletion mutant strain.
