MOESM8 of p66ShcA functions as a contextual promoter of breast cancer metastasis

Additional file 8: Figure S8. ShcA p66 is dispensable for invadopodia formation and gelatin degradation. 488 fluorescently-labeled degradation is represented by signal void. (A) Total surface area degraded (μm2) was determined from images taken from three independent experiments (n = 120 FOV, n = 30 each for 4T1-537 (par), p66CR (VC), p66CR (WT) and p66CR (S36A)) and error bars represent s.e.m. (B) Punctate and Large gelatin degradation patches, representative images shown, were classified qualitatively. Frequency of degradation pattern taken from quantified images used for (A). (C) and (D), group the quantified total surface area degraded (μm2) per FOV, from (A), into Punctate and Large degradation patterns, respectively. (E) Representative images of quantified gelatin degradation: F-Actin (green) stained by 647-phalloidin and 488-labelled gelatin (grey). Scale bar is 20 μm in length. Statistical analysis performed using a one-way Anova with a Tukey’s multiple comparisons test (*P < 0.05; **P < 0.01; ****P < 0.0001).