MOESM8 of Wild-type Cu/Zn-superoxide dismutase is misfolded in cerebrospinal fluid of sporadic amyotrophic lateral sclerosis

Additional file 8: Figure S7. Effects of metal salts addition on the viability of differentiated NSC-34 cells. Differentiated NSC-34 cells were first prepared by the method described in the text, and either CuSO4 or ZnSO4 in the indicated concentration was then added. After incubation for 48 h, the viability of the NSC-34 cells was assayed with Cell Counting Kit-8 (Dojindo) and represented as the relative viability to the one in the absence of the metal salt addition. The data were represented as the averaged cell viability relative to that of the negative control in which neither CuSO4 nor ZnSO4 was added. Three independent experiments were performed to estimate error bars (standard deviation).