12943_2019_1105_MOESM8_ESM.pdf (117.79 kB)
MOESM8 of LncRNA LINRIS stabilizes IGF2BP2 and promotes the aerobic glycolysis in colorectal cancer
journal contribution
posted on 2019-12-03, 04:41 authored by Yun Wang, Jia-Huan Lu, Qi-Nian Wu, Ying Jin, De-Shen Wang, Yan-Xing Chen, Jia Liu, Xiao-Jing Luo, Qi Meng, Heng-Ying Pu, Ying-Nan Wang, Pei-Shan Hu, Ze-Xian Liu, Zhao-Lei Zeng, Qi Zhao, Rong Deng, Xiao-Feng Zhu, Huai-Qiang Ju, Rui-Hua XuAdditional file 8: Figure S3, related to Fig. 3. LINRIS is involved in the autophagic degradation of IGF2BP2. (A) Western blotting shows the levels of IGF2BP2 in the indicated cells with the knockdown of LINRIS after treatment with or without 3-MA (10 mg/ml) for 24 h. GAPDH was used as the loading control. (B) Confocal microscopy of HCT116 cells treated with or without EBSS for 3 h. Scale bar, 50 μm. (C) Western blotting shows the knockout of ATG5 in DLD-1 cells with sgRNA (sg-ATG5) compared with that in control cells. GAPDH was used as the loading control. (D) DLD-1 cells with the knockout of ATG5 and control cells were transfected with shRNAs specific for LINRIS. Cell lysates were analyzed by immunoblotting with GAPDH as the loading control.
