MOESM8 of KIF5B modulates central spindle organization in late-stage cytokinesis in chondrocytes

Additional file 8: Figure S6. Reduced tubulin intensity in midbody in Kif5b knockdown ATDC5 cells. (A) Immunofluorescence of α-tubulin in sh-ctl and sh-Kif5b cells in late cytokinesis. Yellow arrows denote midbody regions. Scale bar: 10 μm. (B) Quantification of tubulin intensity in midbody in both sh-ctl (n = 63 cells from sh-ctl clone #1–3) and sh-Kif5b cells (n = 98 cells from sh-Kif5b clone #4, #5 and #8). ***P < 0.0001; two-tailed Mann-Whitney U-test. The whisker plot shows median (lines), interquartile range (boxes) and 10% to 90% percentile (whiskers).