MOESM8 of Characterization of LrgAB as a stationary phase-specific pyruvate uptake system in Streptococcus mutans

Additional file 8: Figure S8. The effect of protonophores CCCP and DNP on lrg promoter (PlrgA) activity. The PlrgA-gfp reporter strain was grown in a low-glucose (11 mM) FMC medium (A), supplemented by 1 μM CCCP (carbonyl cyanide m-chlorophenyl hydrazine, B) and 0.1 mM DNP (2,4-dinitrophenol, C). Relative gfp expression (green square) and OD600 (grey circle; OD) were monitored on a plate reader (see Materials and Methods for details). The results are representative of two independent experiments.