MOESM7 of Wild-type Cu/Zn-superoxide dismutase is misfolded in cerebrospinal fluid of sporadic amyotrophic lateral sclerosis

Additional file 7: Figure S6. Addition of metal salts has little effects on amounts of SOD1 adsorbed on wells of an ELISA plate. The experiments were performed as described in Fig. 8B except the capture antibody. Briefly, the CSF samples (20 μg of total proteins, 100 μL of total volume) were first incubated with either (blue bars) 100 μM CuSO4 or (red bars) 100 μM ZnSO4 at 4 °C overnight and then analyzed by sandwich ELISA with FL-154 and Pan-SOD1 as capture and detection antibodies, respectively. As a control, the CSF samples incubated at 4 °C overnight without addition of any metal ions (black bars) were also examined. The experiments were performed in triplicate to estimate error bars (standard deviation).