MOESM7 of SELEX tool: a novel and convenient gel-based diffusion method for monitoring of aptamer-target binding

Additional file 7: Figure S6. There is no binding signal in aptamer-acetamiprid by single diffusion. Concentration of Apt: 0.25 μM. Targets concentration: 8.95 mM. Diffusion time was 16 h at room temperature. Binding buffer as a negative control. The abnormal high intensity spot at the top left well was caused by a bubble.