MOESM7 of Reinvestigating the early embryogenesis in the flatworm Maritigrella crozieri highlights the unique spiral cleavage program found in polyclad flatworms

Additional file 7. Cytoplasmic perturbations were imaged with the OpenSPIM in one of the second quartet macromeres during mitosis. On the left the whole embryo is shown with increased brightness to visualize the membranous outlines of the macromeres better. To the right of each embryo, the nuclei of the same embryo are depicted with normal brightness levels. Red arrows point to the same nucleus of the embryo. A red line highlights the outline of the corresponding macromere. The shape deformations caused by the cytoplasmic perturbations of the macromere correlate precisely with the mitotic anaphase and reach a maximum in panel D. Scale bar = 50 µm.