13567_2019_733_MOESM7_ESM.mp4 (17.59 MB)
MOESM7 of Precision cut lung slices: a novel versatile tool to examine host–pathogen interaction in the chicken lung
media
posted on 2020-01-11, 05:08 authored by Karen Bryson, Damien Garrido, Marco Esposito, Gerry McLachlan, Paul Digard, Catherine Schouler, Rodrigo Guabiraba, Sascha Trapp, Lonneke VerveldeAdditional file 7. Dynamic interaction of mononuclear phagocytes with fluorescent latex beads. PCLS (500 μm) prepared from CSF1R-eGFP transgenic chickens were incubated with 1 μm fluorescent latex beads at 3 days post slice, and were examined by time-lapse imaging using a Zeiss Live cell observer with a ×10 objective lens. The PCLS was imaged every 3 min for 18 h. The image was cropped to a region of interest demonstrating that CSF1R-eGFP+ cells (green) were phagocytosing latex beads (red).
