MOESM7 of Precision cut lung slices: a novel versatile tool to examine host–pathogen interaction in the chicken lung
2020-01-11T05:08:38Z (GMT) by
Additional file 7. Dynamic interaction of mononuclear phagocytes with fluorescent latex beads. PCLS (500 μm) prepared from CSF1R-eGFP transgenic chickens were incubated with 1 μm fluorescent latex beads at 3 days post slice, and were examined by time-lapse imaging using a Zeiss Live cell observer with a ×10 objective lens. The PCLS was imaged every 3 min for 18 h. The image was cropped to a region of interest demonstrating that CSF1R-eGFP+ cells (green) were phagocytosing latex beads (red).