MOESM7 of KIF5B modulates central spindle organization in late-stage cytokinesis in chondrocytes

Additional file 7: Figure S5. Failure of maintaining of proper cell polarity in Kif5b deficient chondrocytes. (A) Representative images of GM130 immunofluorescence on the growth plate. In normal proliferative chondrocytes, Golgi apparatus (marked by GM130 with red fluorescence) localizes to one or two sides of the nucleus (marked with DAPI). The cell plane is perpendicular to the longitudinal axis of the growth plate (upper). But in KIF5B depleted chondrocytes, the cells are abnormal shaped. Golgi complex scatters around the cytoplasm, with the cell planes abnormally aligned, compared to the longitudinal axis of the growth plate (lower). (B) Representative images of acetylated-α-tubulin immunofluorescence on the growth plates. It is shown that most normal cells display cilia when stained with the antibody for acetylated-α-tubulin (upper). As well, cilia are preferentially located on the inferior/superior surfaces of the flattened chondrocytes. However, although the epiphyseal chondrocytes in the mutant growth plate are less affected, the proliferative chondrocytes are nearly devoid of cilia, with the acetylated tubulin scattered in the whole cell (lower). Scale bar: 10 μm.