13227_2020_147_MOESM7_ESM.docx (80.95 kB)
MOESM7 of Elongation during segmentation shows axial variability, low mitotic rates, and synchronized cell cycle domains in the crustacean, Thamnocephalus platyurus
journal contribution
posted on 2020-01-19, 04:35 authored by Savvas Constantinou, Nicole Duan, Lisa Nagy, Ariel Chipman, Terri WilliamsAdditional file 7. Correlation of pH3 and Hoechst mitosis counts and cell cycle expression. A. pH3 and Hoechst count correlation for 6 EN animals. We find low correlation at all developmental stages. B. Expression of growth zone pH3 and Hoechst in relation to cell cycle progression. Although pH3 is reported to be expressed throughout M-phase ([37, 73]; red line), we find Thamnocephalus pH3 to be expressed early in M-phase (red dotted line). By comparison, mitosis counts using Hoechst only score cells in late M-phase.