MOESM7 of Effects of cigarette smoke on barrier function and tight junction proteins in the bronchial epithelium: protective role of cathelicidin LL-37

Additional file 7: CSE-induced phosphorylation of ERK and effects of LL-37 on phosphorylation of EGFR and ERK. A. Representative western blots showing phosphorylated EGFR, EGFR, phosphorylated ERK or ERK in Calu-3 cells exposed to 10% CSE for 0, 5 or 10 min after CSE exposure. B. Representative western blots showing phosphorylated EGFR, EGFR, phosphorylated ERK or ERK in Calu-3 cells pretreated with or without 20 μg/ml LL-37 and then exposed to 10% CSE for 5 or 10 min after CSE exposure. Band intensity was quantitated using densitometry. All results are representative of at least two independent experiments. Data represent means ± SEM (n = 4 per group). Differences in data were analyzed by two-way ANOVA.