MOESM6 of Wild-type Cu/Zn-superoxide dismutase is misfolded in cerebrospinal fluid of sporadic amyotrophic lateral sclerosis

Additional file 6: Figure S5. Reactivities of SOD1 antibodies toward conformationally distinct states of SOD1 in vitro were examined by indirect ELISA. Recombinant SOD1 proteins (black, wild-type: white, A4V: red, G37R: blue, G85R) were prepared in the following states: +Cu/Zn S-S, SOD1 with the disulfide bond (SOD1S-S) in the presence of copper and zinc ions; Apo S-S, SOD1S-S in the absence of any metal ions; Apo SH, SOD1 without the disulfide bond (SOD1SH) in the absence of any metal ions; SS oligomer, SOD1 oligomers crosslinked via disulfide bonds prepared from Apo S-S; Aggregates, insoluble SOD1 aggregates prepared from Apo SH. The experimental methods to prepare those SOD1 proteins can be found in our previous papers (ref #7, 8). Proteins (5 μg) were first adsorbed in wells of an ELISA plate and then detected with 0.2 μg/mL of (A) UβB, (B) EDI, (C) SOD1int, (D) apoSOD, (E) 24–39, and (F) Pan-SOD1 antibodies. A detailed procedure for indirect ELISA can be found in our previous papers (ref #35, 36), which have also reported the data on UβB, EDI, SOD1int, and apoSOD. Three independent experiments were performed to estimate error bars (standard deviation).