MOESM6 of Transcriptional induction of capsidiol synthesis genes by wounding can promote pathogen signal-induced capsidiol synthesis

Additional file 6: Figure S4. Transcript levels of GUS fused to the 5′-untranslated region of EAS4 or a 35S minimal promoter. Agrobacterium cells carrying GUS fused to the EAS4 promoter fragments, 5′-untranslated region of EAS4 or a 35S minimal promoter were mixed with those carrying LUC driven by a 35S promoter, and infiltrated into N. benthamiana leaves. At 40–48 h after infiltration, the leaves were wounded, and harvested at the times indicated after wounding. Transcript levels of GUS, LUC, and Nbactin2 were quantified by RT-qPCR, and the level of GUS was doubly normalized to the levels of Nbactin2 and LUC as internal and infection standards, respectively. Values are means with standard deviations of three biological replicates.