MOESM6 of SELEX tool: a novel and convenient gel-based diffusion method for monitoring of aptamer-target binding
2020-01-14T05:16:59Z (GMT) by
Additional file 6: Figure S5. Characterization of aptamer-target binding by single diffusion in mini-gels. A and B, Binding signal of streptavidin (SA) and biotinylated oligodeoxynucleotides (Bio-ODN) (A) and control DNA (B). C, Analysis of diffusion radius centered at the centre of the well for SA. D and E, Binding signal of thrombin and its aptamer (TBA) (D) and control DNA (E). F, Analysis of diffusion radius centered at the centre of the well for thrombin. Arrow heads in A: diffusion ring; Thin arrow heads in D: diffusion trace. Data represent mean ± SEM. *P < 0.05, **P < 0.01, set control = 1.0. GelRed as the DNA indicator.