Springer Nature
Browse
13036_2019_223_MOESM6_ESM.pdf (99.04 kB)

MOESM6 of SELEX tool: a novel and convenient gel-based diffusion method for monitoring of aptamer-target binding

Download (99.04 kB)
journal contribution
posted on 2020-01-14, 05:16 authored by Qingxiu Liu, Wei Zhang, Siying Chen, Zhenjing Zhuang, Yi Zhang, Lingli Jiang, Jun LIN
Additional file 6: Figure S5. Characterization of aptamer-target binding by single diffusion in mini-gels. A and B, Binding signal of streptavidin (SA) and biotinylated oligodeoxynucleotides (Bio-ODN) (A) and control DNA (B). C, Analysis of diffusion radius centered at the centre of the well for SA. D and E, Binding signal of thrombin and its aptamer (TBA) (D) and control DNA (E). F, Analysis of diffusion radius centered at the centre of the well for thrombin. Arrow heads in A: diffusion ring; Thin arrow heads in D: diffusion trace. Data represent mean ± SEM. *P < 0.05, **P < 0.01, set control = 1.0. GelRed as the DNA indicator.

Funding

National Key R&D Program of China

History