MOESM6 of Parallel substrate supply and pH stabilization for optimal screening of E. coli with the membrane-based fed-batch shake flask
2018-05-09T05:00:00Z (GMT) by
Additional file 6. Screening for adequate ammonium carbonate concentrations in addition to 750 g/L glucose in the reservoir for the multi-component release with the membrane-based fed-batch shake flask. Cultivation conditions: Wilms-MOPS-mineral medium (culture medium: 200 mM MOPS with 7 g/L initial ammonium sulfate in culture medium, reservoir: 750 g/L glucose, varied ammonium carbonate concentration, 17.14 g/L K2HPO4 and blue dextran concentration: 1 g/L), temperature: 37 °C, shaking frequency: 350 rpm, shaking diameter: 50 mm, initial culture medium volume: 10 mL, inoculation OD600: 0.5, reservoir filling volume: 2 mL, dialysis membrane: Reichelt 10-20 kDa, membrane area: 18.1 mm2; Strain: E. coli BL21 (DE3) pRhotHi-2-EcFbFP; Concentration values have been volume-corrected; The arrows point to the occurrence of abrupt OTR increases.
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