MOESM6 of Nanobodies targeting conserved epitopes on the major outer membrane protein of Campylobacter as potential tools for control of Campylobacter colonization
2017-12-08T05:00:00Z (GMT) by
Additional file 6. Alignment of amino acid sequences of the MOMP-encoding porA -gene. The porA gene of the C. jejuni and C. coli isolates (Table 1) was amplified using PCR and aligned, using C. jejuni KC40 as a reference strain, to identify conserved regions. The PCR was performed with the primers F3 (5′-ATGAAACTAGTTAAACTTAGTTTA-3′) and R3 (5′-GAATTTGTAAAGAGCTTGAAG-3′). External loops are labelled from L1 to L7 and β-strands are underlined, based on the MOMP structure determined by Ferrara et al. . Loops L3 and L6 are the most conserved in sequence and number of amino acids.
CC BY + CC0