MOESM6 of Induction of LEF1 by MYC activates the WNT pathway and maintains cell proliferation

Additional file 6: Figure S6. (A) RKO and Huh7 cells were transfected with control or LEF1 siRNAs for 3 days and then Western blotted. (B) 5000 ARPE-19 cells with or without MYC overexpression were plated in 12-well plates and incubated with different concentrations of ICG-001 in triplicate for 7 days. Cells were then stained with crystal violet, and the relative viabilities were qualified with ImageJ. (C) RT-qPCR for LEF1 in APRE cells infected with empty vector transfected with LEF1 siRNA.