MOESM6 of Identification and characterization of a new type of inhibitor against the human immunodeficiency virus type-1 nucleocapsid protein

Additional file 6: Figure S5. Induction of hyper-stable core of HIV-1 by A1752. MT4 cells were infected with HIV-1 NL4-3/EGFP virus together with the A1752 and Tenofovir treatment at an increasing concentration. The released virus particles were permeabilized with melittin at indicated amounts, followed by incubation at 37 °C for 30 min. The pellet and supernatant fraction were analyzed using western blot assays with anti-CA antibodies.