MOESM6 of A rapid passage through a two-active-X-chromosome state accompanies the switch of imprinted X-inactivation patterns in mouse trophoblast stem cells

Additional file 6: The two-active-X state of early HAT resistant clones is not associated with cell reprogramming towards pluripotency. A. Cumulative histograms showing the expression levels of pluripotency markers Nanog and Oct3/4 measured by RT-qPCR in F3 parental female TS cells and in 3 clones harbouring two active X-chromosomes. Expression levels in XX ES cells (LF2 cell line) are shown for comparison. Standardisation by Rplp0 has been applied. A.U.: Arbitrary Units. B. Same as in panel A on individual cells of each population. Small horizontal bars represent median values. See Additional file 7 for raw quantifications. C. Cumulative histograms showing the level of expression of TS specific markers Cdx2 and Eomes and of markers of TS differentiated states, Esx1 and Prl3b1 [19], assessed by RT-qPCR on a the same clones as in panel A. Expression levels have been standardised by Rplp0. Expression levels measured in F3 TS cells and in F3 TS cells differentiated for 5 days are shown for comparison. A.U. Arbitrary Units. Log scale is used. D. Same as in panel C for Cdx2 and Eomes on individual cells of each population. Small horizontal bars represent median values. See Additional file 7 for raw quantifications.