MOESM6 of A rapid passage through a two-active-X-chromosome state accompanies the switch of imprinted X-inactivation patterns in mouse trophoblast stem cells
2015-12-01T05:00:00Z (GMT) by
Additional file 6: The two-active-X state of early HAT resistant clones is not associated with cell reprogramming towards pluripotency. A. Cumulative histograms showing the expression levels of pluripotency markers Nanog and Oct3/4 measured by RT-qPCR in F3 parental female TS cells and in 3 clones harbouring two active X-chromosomes. Expression levels in XX ES cells (LF2 cell line) are shown for comparison. Standardisation by Rplp0 has been applied. A.U.: Arbitrary Units. B. Same as in panel A on individual cells of each population. Small horizontal bars represent median values. See Additional file 7 for raw quantifications. C. Cumulative histograms showing the level of expression of TS specific markers Cdx2 and Eomes and of markers of TS differentiated states, Esx1 and Prl3b1 , assessed by RT-qPCR on a the same clones as in panel A. Expression levels have been standardised by Rplp0. Expression levels measured in F3 TS cells and in F3 TS cells differentiated for 5Â days are shown for comparison. A.U. Arbitrary Units. Log scale is used. D. Same as in panel C for Cdx2 and Eomes on individual cells of each population. Small horizontal bars represent median values. See Additional file 7 for raw quantifications.