13058_2020_1245_MOESM5_ESM.pdf (9.21 MB)
MOESM5 of p66ShcA functions as a contextual promoter of breast cancer metastasis
journal contribution
posted on 2020-01-16, 04:56 authored by Kyle Lewis, Alex Kiepas, Jesse Hudson, Julien Senecal, Jacqueline Ha, Elena Voorand, Matthew Annis, Valerie Sabourin, Ryuhjin Ahn, Rachel Selva, Sébastien Tabariès, Brian Hsu, Matthew Siegel, Matthew Dankner, Eduardo Canedo, Mathieu Lajoie, Ian Watson, Claire Brown, Peter Siegel, Josie Ursini-SiegelAdditional file 5: Figure S5. p66ShcA does not alter the mesenchymal properties of 4T1-derived triple negative breast cancers. (A) Immunoblot analysis of whole cell lysates isolated from 4T1-537 parental, p66-CR (VC), p66-CR (WT) and p66-CR (S36A) mammary tumors (n = 18 each) using ShcA-, E-Cadherin, Vimentin and Tubulin-specific antibodies. (B-D) Densitometric quantification of mammary tumors shown in panel A for the (B) p66ShcA/Tubulin, (C) p66ShcA/p52ShcA, (D) E-Cadherin/Tubulin and (E) Vimentin/Tubulin ratios. The data is normalized to the parental 4T1-537 tumors.
