MOESM5 of TSG-6 in conditioned media from adipose mesenchymal stem cells protects against visual deficits in mild traumatic brain injury model through neurovascular modulation

Additional file 5: Figure S5. Differential gene expression in blast injury mice without and with primed siControl-ASC-CCM and primed siTSG-6-ASC-CCM. (A): Heatmap representing the directed global significance score for the represented group comparisons calculated as the square root of the mean signed squared t-statistic for the genes in a gene set, with t-statistics coming from the linear regression underlying the differential expression analysis. (B): Volcano plot displaying each gene’s -log10 (p-value) and log2 fold change with the selected covariate. Highly statistically significant genes fall at the top of the plot above the horizontal lines, and highly differentially expressed genes fall to either side. Horizontal lines indicate various False Discovery Rate (FDR) thresholds or p-value thresholds if there is no adjustment to the p-values. Genes are colored if the resulting p-value is below the given FDR or p-value threshold. The 40 most statistically significant genes are labeled in the plot. (C): Representative violin plots of differentially expressed genes in all four study groups. (D): Venn diagram showing significant differentially expressed genes. Data represent combined averages from n = 4–9 animals/group using NanoString Advanced Analysis.