MOESM5 of Septin and Ras regulate cytokinetic abscission in detached cells

Additional file 5: Figure S3. Active Ras, but not SV40 LT protein, promotes tubulin bundling and the recruitment of CHMP4B to the MB in detached cells. Representative immunofluorescence images illustrating the presence of α-tubulin (red) and CHMP4B (green) at the ICB and MB, respectively, in mitotic BJ-LT (A) and BJ-LT-Ras cells (C) after culture for 1 h on fibronectin or in suspension. (B, D) Mean% ± SD of the number of cells having CHMP4B at the MB. The square frames show the midbody region at higher magnification.