MOESM5 of SELEX tool: a novel and convenient gel-based diffusion method for monitoring of aptamer-target binding

Additional file 5: Figure S4. There are no binding signals in aptamer- profenofos (A) and -acetamiprid (B) by double diffusion, respectively. Concentration of Aptamers: 10 μM. Targets concentration: profenofos,10 mM; acetamiprid, 9.85 mM. Diffusion time was 6 h at room temperature. Binding buffer as a negative control.