MOESM5 of Receptor kinase FERONIA regulates flowering time in Arabidopsis

Additional file 5: Figure S5. The relative mRNA levels of unspliced FLC intron 1 and intron 6 decreased in fer-4 mutant. Total RNA were extracted from 7-d-old seedling grown under LD condition and collected at ZT 12. The primer pairs F1/R1’ and F6’/R6 were used to detect the unspliced RNA for FLC introns 1 and 6, respectively. Primer pairs F1/R1 and F6/R6 were used to detect the spliced mRNA. The experiments were performed three times the bar indicates the mean ± SD (**P < 0.01, Student’s t-test).