MOESM5 of Recapitulation of gametic DNA methylation and its post-fertilization maintenance with reassembled DNA elements at the mouse Igf2/H19 locus
2020-01-15T05:12:58Z (GMT) by
Additional file 5: Figure S5. DNA methylation status of the LCb and LCb118 fragments in somatic cells of YAC-TgM. (A and C) Partial restriction enzyme maps of the β-globin YAC transgenes with the inserted LCb (A) or LCb118 (C) fragments. Methylation-sensitive BstUI sites in BamHI fragments are displayed as vertical lines beneath each map. (B and D) DNA methylation status of the LCb (B) or LCb118 (D) fragments in tail somatic cells of the YAC-TgM. Tail genomic DNA was digested with BamHI alone (B) or BamHI + BstUI (B + BstUI) and the Southern blots were hybridized with the probe shown in the maps (A and C). Asterisks indicate the positions of parental or methylated, undigested fragments. ID numbers of individuals inheriting the transgene maternally and paternally are highlighted in pink and blue colors, respectively. In the pedigree, male and female individuals are represented as rectangles and circles, respectively. Filled, gray, or open symbols indicate hyper-, partially-, or hypo-methylated status of LCb or LCb118 fragment in each TgM, which was independently determined by visual examination of the Southern blot results by three individuals. Tail DNA from underlined animals (in the pedigree) was pooled according to the transgene’s parental origin and analyzed by bisulfite sequencing in Fig. 5b, c. Testis samples in Additional file 6: Fig. S6 were obtained from male individuals marked by stars.