MOESM5 of Rapid generation and selection of Cas9-engineering TRP53 R172P mice that do not have off-target effects
datasetposted on 09.11.2019 by Guoxing Zheng, Qingqing Zhu, Junchao Dong, Xin Lin, Chengming Zhu
Datasets usually provide raw data for analysis. This raw data often comes in spreadsheet form, but can be any collection of data, on which analysis can be performed.
Additional file 5: Table S1. Summary of the analysis of the potential off-target loci. The top 10 potential off-target loci are PCR amplified and subsequently subjected to Sanger sequencing and aligned with mouse genome. Although no off-target effects of #2â€“10 loci are found on all the 4 mice, the off-target effects of #1 locus are detected in KI1 and KI3 mice.