MOESM5 of MicroRNA-155 contributes to shear-resistant leukocyte adhesion to human brain endothelium in vitro
2019-10-10T08:55:41Z (GMT) by
Additional file 5: Video S4. Shear-resistant firm adhesion of THP1 cells to CONTROL Scrambled-Pre-miR transfected and unstimulated hCMECD3 cells. THP1 cells were pulled through the channel over hCMEC/D3 monolayers under low shear (0.5 dyn/cm2). After 5 min, flow shear stress was increased (1.5 dyn/cm2) to challenge non-firmly adherent THP1. The number of arrested THP1 constantly increased during the accumulation phase and only the one that remained stationary on the endothelial monolayer were manually counted in 5 different FOV along the channel of Ibidi m-Slide VI. FITC channel was used to count fluorescently firmly adhered THP1 cells. Objective 10x, at 40 images per min (1 phase-contrast and 1 FITC), field of view 640 μm × 480 μm, recording time 5.5 min.