13567_2019_676_MOESM5_ESM.tif (2.28 MB)
MOESM5 of Intraepithelial neutrophils in mammary, urinary and gall bladder infections
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posted on 2019-07-20, 04:21 authored by Dvir Mintz, Hagit Salamon, Michal Mintz, Ilan Rosenshine, Nahum ShpigelAdditional file 5. Live neutrophil (white arrow in A) in mammary epithelial cell enclosed in a double membrane compartment tethered to epithelial cell junctional complex. Lactating C57BL/6 mice where infused with 106 CFUs of viable E. coli DH5Îą bacteria. Transmission electron microscopy imaging of mammary tissues 24 hours after challenge. Boxed areas in A is enlarged in B and boxed areas in B are enlarged in C, D). Tethering of neutrophil to epithelial junctional complex is visible (black arrows in B, D). Microvilli and milk space (black asterisk * in C) are visible adjacent to the epithelial cell junctional complex (Black arrow in C). Tethering of double membrane to the basolateral membrane of host epithelial cell is also visible (yellow arrows in B and D). Scale bars 2000 nm (A), 1000 nm (B), and 500 nm (C, D).
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transmission electron microscopy imagingScale bars 2000 nmBlack arrow5. Live neutrophilIntraepithelial neutrophilsepithelial cell junctionalLactating C 57BL micemembrane compartment500 nmtissues 24 hourscoli DH 5Îą bacteriabasolateral membranegall bladder infectionsepithelial cellmilk spaceTetheringMOESM 5Boxed areas106 CFUsepithelial junctionalhost epithelial cell
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