MOESM5 of Evaluation of scFv protein recovery from E. coli by in vitro refolding and mild solubilization process
2019-01-14T05:00:00Z (GMT) by
Additional file 5: Figure S5. Periplasmic expression pattern of scFv antibody protein in fusion with MBP tag. MBP.scFv-pMAL-p5X recombinant plasmid was used to transform E. coli BL21 bacteria and cells were grown up to OD (A600 ~ 0.5) followed by expression with 0.3 mM IPTG concentration for 2 h at 37 °C. Harvested cells were used for protein extraction following periplasmic extraction method. The protein isolated from both in supernatant (S) and pellet (P) was used to separate by SDS 12 % polyacrylamide gel electrophoresis. Un-induced transformed cells were also extracted by periplasmic extraction method and also allowed for SDS 12 % polyacrylamide gel electrophoresis as control (C) sample.
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