MOESM5 of Effects of cigarette smoke on barrier function and tight junction proteins in the bronchial epithelium: protective role of cathelicidin LL-37

Additional file 5: No protective effects of LL-37 on CSE-induced reduction in TEER disappeared at 48 h after CSE exposure. Calu-3 cells pretreated with or without 20 μg/ml LL-37 were exposed to 10% CSE. A. TEER was measured at 48 h after CSE exposure without retreatment with LL-37. B. Retreatment with 20 μg/ml LL-37 without replacement of the medium was performed at 24 h after CSE exposure and then TEER was measured at 48 h. All results are representative of at least two independent experiments. Data reresent means ± SEM (n = 6–8 per group). *p < 0.001 by one-way ANOVA.