MOESM5 of EFTUD2 gene deficiency disrupts osteoblast maturation and inhibits chondrocyte differentiation via activation of the p53 signaling pathway

Additional file 5: Figure S4. EFTUD2 gene knockdown in HC-a. A: Expression of EFTUD2 mRNA in HC-a cells transfected with sh2 and sh3 lentivirus was lower than that in the shNT control.B: Protein expression of EFTUD2 decreased in HC-a cells transfected with sh2 and sh3 lentivirus. C: Cell proliferation of HC-a cells transfected with sh2 and sh3 lentivirus was disrupted compared with that of the shNT control. D-E: COL10A1 mRNA levels among different groups (shNT, sh2, sh3) of HC-a cells at 3 days before cell confluence, 3 days after cell confluence and 2 weeks after cell confluence. F-G: SOX9 mRNA levels in HC-a cells among different groups (shNT, sh2, sh3) at 3 days before and after cell confluence. H: Alcian blue staining of HC-a cells among different groups (transfected with shNT, sh2 and sh3 lentiviruses). *: P < 0.05, **: P < 0.01, ***: P < 0.001.