MOESM5 of DUF3669, a “domain of unknown function” within ZNF746 and ZNF777, oligomerizes and contributes to transcriptional repression
2019-12-20T06:34:45Z (GMT) by
Additional file 5. Verification of expression and expected size of the Gal4 fusion proteins encoded by the constructed pM3 expression vectors using Western blotting in HeLa cells (a, b, c, d, e, f) and HAP1 wild type cells (g - i). Cell extracts made 24 h post-transfection with 1 x SDS sample buffer were probed with rabbit polyclonal antibodies against GAL4 (upper panels; Santa Cruz Biotechnology sc-577 at 0.2 μg/ml) and monoclonal antibodies against endogenous GAPDH (lower panels; Abcam ab8245 at 0.1 μg/ml). Non-transfected HeLa cell lysates are used as negative controls. Black block arrows point to bands of expected size when more than one protein species is visible; * indicates bands due to cross-reactivity of the antibodies. Panels a-i cover all Gal4 fusion protein constructs used in the manuscript.