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MOESM5 of Characterization of the BspA and Pmp protein family of trichomonads

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posted on 2019-08-20, 12:01 authored by Maria Handrich, Sriram Garg, Ewen Sommerville, Robert Hirt, Sven Gould
Additional file 5: Figure S5. Localizations were further verified by using different detergents. The proteins were localized by a specific antibody against the HA tag (green), the nucleus was detected by using 4′,6-diamidin-2-phenylindol (DAPI; blue). Except for the N-terminal tagged Pmp (TVAG_212570) and BspA (TVAG_240680) protein, which were again found to reside inside a single enlarged lysosome, all the other constructs analyzed show the same ER/Golgi localization. Scale-bar: 5 µm.

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