MOESM5 of A novel gene from a secondary metabolism gene cluster is required for microsclerotia formation and virulence in Verticillium dahliae

Additional file 5: Figure S5. Expression of Nag1 is regulated by the MADS-box transcription factor VdMcm1. a RNA-Seq coverage data showed the reduction in Nag1 transcript abundance in the VdMcm1 deletion mutant relative to WT. The novel transcript is indicated by a dotted red box. b Relative expression level of Nag1 in the VdMcm1 deletion mutant. Relative transcript levels were determined by qRT-PCR using the ΔΔCT method, with β-tubulin as a reference. Error bars indicate standard deviation from three independent experiments. (JPG 358 kb)