MOESM5 of A long noncoding RNA promotes cellulase expression in Trichoderma reesei
2018-03-23T05:00:00Z (GMT) by
Additional file 5. RNA stability of the three HAX1 versions. The T. reesei strains QM6a (blue), QM9414 (yellow) and Rut-C30 (purple) were grown in MA medium containing 1% (w/v) α-d-lactose for 24 h and transcription was inhibited by the addition of DRB. Samples were taken before the addition of DRB (reference sample) and 30 min, 60 min, 90 min and 120 min after the addition of DRB. RT-qPCR was performed using cDNA from QM6a for the amplification of hax1QM6a (primer pair up-hax1 for_2 and hax1 rev_1.Intron, blue), from QM9414 for the amplification of hax1QM9414 (primer pair hax1 for_qPCR_QM9414 and hax1 rev_up-Intron; yellow) and from Rut-C30 for the amplification of hax1Rut-C30 (primer pair up-hax1 for_1 and hax1 rev_up-Intron, purple). Transcript levels were normalized to act and sar1, refer to the sample taken before the addition of DRB (time point 0 min) and are given in logarithmic scale (lg). All standard deviations of technical triplicates are < 0.06.