MOESM4 of Wild-type Cu/Zn-superoxide dismutase is misfolded in cerebrospinal fluid of sporadic amyotrophic lateral sclerosis

Additional file 4: Figure S3. Selectivity of C4F6 antibody toward mutant/misfolded SOD1. Sandwich ELISA was performed as described in the text (see Methods). Briefly, C4F6 (1:500 dilution) antibody was first adsorbed on wells of an ELISA plate, and recombinant apo-SOD1 variants (WT, A4V, G37R, G85R, and G93A) with the disulfide bond (0.1 μg) was then applied to the well. Pan-SOD1 (1:500 dilution) and anti-sheep secondary antibody (1:500 dilution) were used as detection and secondary antibodies, respectively. Three independent experiments were performed to estimate error bars (standard deviation).