MOESM4 of Unbiased phenotypic identification of functionally distinct hematopoietic progenitors

Additional file 4: Figure S3. Identification of colonies based on the expression of CD235a (x axis) and CD41 (y axis). A, B Distinction between erythroid, mixed and megakaryocytic colonies was made based on the ratio between CD41+ cells and CD235a+ cells. Colonies where CD41+ cells were more than CD235a were identified as megakaryocytic while colonies where CD235a+ cells where at least 1.15x more than CD41+ were identified as purely erythroid. Colonies where ratio between CD235a+ cells and CD41+ is proportional, were labelled as mixed. In addition, megakaryocytic colonies were distinguished from mixed on the frequency of CD42+ cells within the CD41+ fraction. Wells with > 80% double negative were identified as GM. C Frequency of each colony type as percentage of total colonies observed.