MOESM4 of Stepwise metabolic engineering of Escherichia coli to produce triacylglycerol rich in medium-chain fatty acids

Additional file 4: Figure S4. A putative acyl-CoA synthetase RoFadD2 from R. opacus PD630 did not increase TAG. Cells were cultured in ZYP-5052 auto-induction medium at 37 °C with shaking at 200 rpm for 48 hr. Lipids extracted from 2.5 mg dried cells were loaded on TLC plate.