MOESM4 of Role of neuromedin B and its receptor in the innate immune responses against influenza A virus infection in vitro and in vivo

Additional file 4. Effect of trypsin on the expression of IL-6, PAR2 and NMBR in vitro. The BMDMs and A549 cells were infected with PR8 (MOI = 1) in the presence or absence of trypsin. The BMDMs and A549 cells were collected at the indicated times to test the expression of IL-6 mRNA by RT-PCR and qRT-PCR and the expression of PAR2 and NMBR by Western blotting. (A, B) IL-6 mRNA expression in BMDMs. (C) PAR2 and NMBR expression in BMDMs. (D) NMBR expression in A549 cells. β-Actin was used as the reference housekeeping gene for internal standardization.