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MOESM4 of Pcdhβ deficiency affects hippocampal CA1 ensemble activity and contextual fear discrimination

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posted on 21.01.2020 by Hirotaka Asai, Noriaki Ohkawa, Yoshito Saitoh, Khaled Ghandour, Emi Murayama, Hirofumi Nishizono, Mina Matsuo, Teruyoshi Hirayama, Ryosuke Kaneko, Shin-ichi Muramatsu, Takeshi Yagi, Kaoru Inokuchi
Additional file 4: Figure S4. Pcdhβ deficiency does not affect the number of ensembles activated in the home cage and during novel context exploration (related to Fig. 2). (a) Schematic diagram of the non-negative matrix factorization (NMF) analysis. (b) Representative image of an ensemble extracted with NMF. Red arrows (left) indicate the cells contributing to this ensemble. Red dots (right) indicate the times at which the ensemble was activated. (c–d) Normalized number of ensembles extracted in each session (panel c: F(4, 3) = 1.907, P = 0.6229; t7 = 1.648, P = 0.1434; panel d: F(4, 3) = 1.086, P = 0.9856; t7 = 0.2852, P = 0.7837; unpaired t-test; n = 5 Wt mice, 4 Δβ mice). Data are means ± SEMs.


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