12969_2020_403_MOESM4_ESM.docx (216.19 kB)
MOESM4 of Patients with enthesitis related arthritis show similar monocyte function pattern as seen in adult axial spondyloarthropathy
journal contribution
posted on 2020-01-16, 04:54 authored by Shruti Bhattacharya, Ramnath Misra, Amita AggarwalAdditional file 4 TNF and IL-6 mRNA fold change (PB) in HC, SpA and ERA patients. Scatter plots representing TNF and IL-6 mRNA fold change in PB in three group of subjects, HC (5), SpA (5) and ERA (5) patients as measured via quantitative PCR Each dot represents an individual sample. Horizontal line represents mean. Fold change =2-ΔΔCt and ΔΔCt = [Ct(TNF/IL-6)-CtGAPDH] stimulated sample (LPS/TNC/MRP8) - [Ct(TNF/IL-6)-CtGAPDH unstimulated sample. TNF mRNA fold change in response to (A). LPS stimulation (B). TNC stimulation (C). MRP8 stimulation. IL-6 mRNA fold change in response to (D). LPS stimulation (E). TNC stimulation (F). MRP8 stimulation. WB diluted 1:1 with complete culture medium was used. HC: healthy controls, SpA: Spondyloarthropathy, ERA: enthesitis related arthritis, Uns- unstimulated, LPS- Lipopolysaccaride, PG- peptidoglycan, TNC- Tenascin-C and MRP8-Myeloid related protein 8, TNF: tumor necrosis factor, IL-6: Interleukin-6.