Springer Nature
Browse
12967_2018_1578_MOESM4_ESM.pptx (817.42 kB)

MOESM4 of PBMC fixation and processing for Chromium single-cell RNA sequencing

Download (817.42 kB)
presentation
posted on 2018-07-17, 05:00 authored by Jinguo Chen, Foo Cheung, Rongye Shi, Huizhi Zhou, Wenrui Lu
Additional file 4: Figure S2. Fixation processing did not aggregate the cells. PBMCs with greater than 2500 genes detected (a) or CD8+ cells with more than 2000 genes detected (b) were flagged because these droplets usually contain more than one cell (doublet or multiplet). Fixation did not increase the rate of these GEMs/cells. Methanol-fixed PBMCs remained microscopically visible as single, intact round cells with the similar size as live ones.

Funding

Division of Intramural Research, National Institute of Allergy and Infectious Diseases

History

Usage metrics

    Journal of Translational Medicine

    Categories

    Keywords

    FixationMethanolSSCPBMCPrimary cellsDroplet-based single-cell RNA-SeqChromium

    Licence

    CC BY + CC0

    Exports

    RefWorks
    BibTeX
    Ref. manager
    Endnote
    DataCite
    NLM
    DC