MOESM4 of PBMC fixation and processing for Chromium single-cell RNA sequencing

Additional file 4: Figure S2. Fixation processing did not aggregate the cells. PBMCs with greater than 2500 genes detected (a) or CD8+ cells with more than 2000 genes detected (b) were flagged because these droplets usually contain more than one cell (doublet or multiplet). Fixation did not increase the rate of these GEMs/cells. Methanol-fixed PBMCs remained microscopically visible as single, intact round cells with the similar size as live ones.