MOESM4 of Leptin decreases the expression of low-density lipoprotein receptor via PCSK9 pathway: linking dyslipidemia with obesity

Additional file 4: Figure S4. Examination of the effect of leptin on PCSK9, LDLR, HNF1α and SREBP2 mRNA Levels in dose- or time-dependent manner in HepG2 cells. (A) Real time PCR analysis of LDLR, PCSK9, HNF1α and SREBP2 mRNA levels in HepG2 cells treated with leptin (0, 5, 25, 50, 100 and ng/mL) for 24 h. (B) Real time analysis of LDLR, PCSK9, HNF1α and SREBP2 mRNA levels in HepG2 cells treated with leptin (50 ng/ml) for 0, 6, 12, 24, 48 h. *p < 0.05 represent significant differences expression compared to the vehicle-treated cells.