MOESM4 of Intraepithelial neutrophils in mammary, urinary and gall bladder infections

Additional file 4. E. coli DH5α is not mammary pathogenic and does not replicate in the mammary gland following intramammary challenge. A, Mammary bacterial burden 24 hours after intramammary challenge with 101 to 106 viable E. coli DH5α into wild-type C57BL/6 mice are shown. Each symbol represents one gland and all bars represent the median. Medians CFU/gr were analyzed using One-Sample Wilcoxon Signed Rank Test and the null hypothesis was median of CFUs/gr equals to the challenge dose (101 to 106), none of which were statistically significant. H&E staining of formalin-fixed mammary tissues (B–D). Mammary gland with massive recruitment of neutrophils into the alveoli (white arrows in B) 24 h after infusion with 106 viable E. coli DH5α. This is better seen in Figure 5A (white arrow), which is an enlargement of the boxed area in C. Scale bar 200 µm (B).