MOESM4 of Impact of mesenchymal stem cells’ secretome on glioblastoma pathophysiology

Additional file 4: Figure S1. mRNA expression of genes coding for proteins detected in proteomic analyses by quantitative RT-qPCR. (A) Gel electrophoresis of CCL2, TPT1, POSTN, TGFβI, SEMA7A, PDGFC, IL6 and TBP expression in HUCPVCs. The RT-qPCR products were run on a 2% agarose gel. (B) Relative mRNA expression quantification in HUCPVCs. Data is normalized for TBP expression, and results are expressed as the mean ± SD of 3 biological replicates. M, Molecular weight marker 100 bp, ThermoScientific; #1, #2, and #3, independent biological replicates of HUCPVCs; (-), negative control.