13071_2019_3375_MOESM4_ESM.tif (588.35 kB)
MOESM4 of Hepatocellular carcinoma-associated antigen 59 of Haemonchus contortus modulates the functions of PBMCs and the differentiation and maturation of monocyte-derived dendritic cells of goats in vitro
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posted on 2019-03-14, 05:00 authored by QiangQiang Wang, LingYan Wu, Muhammad Hasan, MingMin Lu, WenJuan Wang, RuoFeng Yan, LiXin Xu, XiaoKai Song, XiangRui LiAdditional file 4: Figure S2. An immunofluorescence assay was conducted to determine if rHCA59 could bind to goat PBMCs (a) and md-DCs (b). Localization was performed by incubating DCs or PBMCs with rat anti-rHCA59 IgG or negative rat IgG (control). a1, a2, b1, b2 Staining of the target proteins (red) was visualized by Cy3-conjugated secondary antibody. a3, a4, b3, b4 Nuclei of the corresponding cells were visualized by DAPI (blue) staining. a5, a6, b5, b6 Merged, overlap of the red and blue channels. No red fluorescence was observed in the control group. Scale-bars: a, 10 Îźm; b, 5 Îźm.