MOESM4 of Genome editing of oncogenes with ZFNs and TALENs: caveats in nuclease design

Additional file 4: Figure S4. TALEN designed against E6 could not yield editing in both SiHa and Caski cells. (A) Schematic of three TALEN binding sites on E6 gene. (B, C) TALEN targeting sequence at position 284 and its T7E1 assay in SiHa and CaSki cells showed no significant editing. (D, E) TALEN targeting sequence at position 57 and its T7E1 assay in SiHa and CaSki cells showed no significant editing. (F, G) TALEN targeting sequence at position 112 and its T7E1 assay in SiHa and CaSki cells showed no significant editing.